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10 × klenow-fragment reaction buffer  (Thermo Fisher)


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    Thermo Fisher 10 × klenow-fragment reaction buffer
    10 × Klenow Fragment Reaction Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/klenow+reaction+buffer/10+%C3%97+klenow+fragment+reaction+buffer/pmc11649696-149-17-20
    Average 90 stars, based on 1 article reviews
    10 × klenow-fragment reaction buffer - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Circular versus linear RNA topology: different modes of RNA–RNA interactions in vitro and in human cells
    Article Snippet: Unlabelled dNTPs, DNase I, T7 RNA polymerase, Klenow fragment exo-, Klenow reaction buffer, RiboLockTM, RNA size standards, and T4 polynucleotide kinase were purchased from ThermoFisher Scientific (Schwerte, Germany).

    Article Title: Expression profiling of signature gene sets with trinucleotide threading.
    Article Snippet: This reaction was initiated by sequentially adding a 24-μl mixture containing 5 units of an exonucleasedeficient Klenow fragment polymerase (MBI Fermentas, Burlington, ON, Canada) in 1.6× Klenow reaction buffer (80 mM Tris–HCl (pH 8.0), 8 mM MgCl2, and 1.6 mM DTT; MBI Fermentas) with 0.8 μg/μl BSA (New England Biolabs, Ipswich, MA, USA) and a 16-μl solution consisting of 1.875 μM of each nucleotide, 50% of the dCTP and dTTP being Cy5-labeled (GE Healthcare), in 1.25× Klenow reaction buffer (62.5 mM Tris–HCl (pH 8.0), 6.25 mM MgCl2, and 1.25 mM DTT; MBI Fermentas) with 0.6 μg/μl BSA.

    Incubation:

    Article Title: Genomics-Based Molecular Epidemiology of Campylobacter jejuni Isolates from Feedlot Cattle and from People in Alberta, Canada
    Article Snippet: Three microliters of random primers (Invitrogen Corporation, Carlsbad, CA) were added to approximately 6 g of genomic C. jejuni DNA (Cy3 for reference strain NCTC 11168, Cy5 for the test strain) in 1.5-ml amber tubes (Diamed Lab Supplies, Inc., Mississauga, ON, Canada). .. Distilled water was then added so that each tube contained a total of 40.5 l. The contents were then denatured at 95 to 97°C for 6 min, cooled on ice for 2 min, and then left at room temperature for 5 min. Five microliters of 10 Klenow reaction buffer (USB Corporation, Cleveland, OH), 1.5 l Cy-labeled dCTP (Amersham Biosciences, Inc., Sunnyvale, CA), 1 l deoxynucleoside triphosphate (Amersham Biosciences, Inc.), and 20 units exonuclease-free Klenow (USB Corporation) were added and the tubes incubated at 37°C for 2 h. Then, 2.5 l of 0.5 M EDTA was added to each tube and left for 1 min at room temperature. ..



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    Image Search Results


    ( A ) Schematic diagrams depicting the polymerization catalyzed by DNA polymerase I, the ligation catalyzed by T4 DNA ligase and the digestion catalyzed by DNase I, along with comparisons of interfacial reaction efficiency when using AVS versus not using AVS. ( B ) Schematic diagram outlining the iEOS process on AVS. ( C ) Comparison of the stepwise yield, full-length yield, and deletion error rate with and without the use of AVS. ( D ) On the basis of NGS results, a synthetic step-by-step yield was determined through comparison with the target sequence (* P < 0.05; ** P ≤ 0.001; *** P ≤ 0.0001).

    Journal: Science Advances

    Article Title: Scalable acoustic virtual stirrer for enhanced interfacial enzymatic nucleic acid reactions

    doi: 10.1126/sciadv.adt6955

    Figure Lengend Snippet: ( A ) Schematic diagrams depicting the polymerization catalyzed by DNA polymerase I, the ligation catalyzed by T4 DNA ligase and the digestion catalyzed by DNase I, along with comparisons of interfacial reaction efficiency when using AVS versus not using AVS. ( B ) Schematic diagram outlining the iEOS process on AVS. ( C ) Comparison of the stepwise yield, full-length yield, and deletion error rate with and without the use of AVS. ( D ) On the basis of NGS results, a synthetic step-by-step yield was determined through comparison with the target sequence (* P < 0.05; ** P ≤ 0.001; *** P ≤ 0.0001).

    Article Snippet: Following this, a 10-μl polymerization reaction buffer was prepared by mixing Klenow fragment of DNA polymerase (1 U/μl M0210, NEB), 100 μM deoxycytidine triphosphate–Cy3, 5 mM deoxy-ribonucleoside triphosphate (dNTP) mix, 50 mM NaCl, 10 mM tris-HCl, 10 mM MgCl 2 , and 1 mM dithiothreitol (DTT) (pH 7.9).

    Techniques: Ligation, Comparison, Sequencing